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Ascorbate concentrations were expressed as mg 100 g−1 on fresh weight basis. The total phenol content in fruit tissue was estimated using theFolin-Ciocalteu colorimetric method as described by Gonz´alez-Aguilar et al. (2004). For total phenol extraction, 2 g of guava fruit was... 顯示更多 Ascorbate concentrations were expressed as mg 100 g?1 on fresh weight basis. The total phenol content in fruit tissue was estimated using the Folin-Ciocalteu colorimetric method as described by Gonz′alez- Aguilar et al. (2004). For total phenol extraction, 2 g of guava fruit was homogenized with 5mL of ethanol with a tissue homogenizer (Model Virtis, USA). After 2 h in the dark, the mixture was filtered using Whatman paper No. 1. The reaction mixture consisted of 40 _L of the total phenol extract, 50 _L ethanol, 400_L water, and 5_L of a solution containing 2 g of sodium carbonate in 100mL 0.1N NaOH and 500 _L of half strength Folin-Ciocalteu reagent. After 30 min of reaction time, the absorbance of solution was read in a spectrophotometer set at 724 nm and concentrations were calculated by preparing a gallic acid standard curve. 2.6. Skin colour index Subjective rating of skin colour of guava fruit was done after 30 days storage on a 1–5 scale where: 1, 80% yellow. These colour stages were even found useful to define the ripening stage in the coloured cultivar, ‘Apple Colour’ as the background colour dominated. 更新: 不要翻譯軟體直接翻的喔>
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維生素C濃度,表示為毫克100克- 1的基礎上鮮重。 總酚含量在水果組織估計使用 福林一Ciocalteu比色法所描述的Gonz'alez - 阿吉拉等人。 ( 2004年) 。 對於總酚提取2克,番石榴果實製成的5mL乙醇與組織勻漿(型號可見光,美國) 。 2小時後,在黑暗中的 篩選的混合物是使用瓦特曼1號文件。反應 混合組成的40 _L的總酚提取物, 50 _L 乙醇, 400_L水, 5_L解決含2克 碳酸鈉在100毫升0.1N氫氧化鈉和500 _L的一半 實力福林一Ciocalteu試劑。 經過30分鐘的反應時間, 吸光度的解決辦法是在讀光譜儀一套 在724 nm和濃度計算的準備 沒食子酸標準曲線。 2.6 。膚色指數 主觀評價膚色的番石榴果實做 在30天之後,儲存在1-5個規模: 1 , “ 20 %的黃色; 2 ,黃色的20-40 % ; 3 ,黃色的40-60 % ; 4 ,黃色的60-80 % ; 5 , “ 80 %的黃色。這些膚色階段,甚至找到有用的 確定了成熟階段,有色品種,蘋果色' 作為背景顏色為主。
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